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KMID : 1007520170260030731
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Food Science and Biotechnology
2017 Volume.26 No. 3 p.731 ~ p.738
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Improved synthesis of isomaltooligosaccharides using immobilized α-glucosidase in organic?aqueous media
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Wang Jun
Li Wei
Lu Fu Ping
Singh Suren
Niu Dan Dan
Liu Xiao Guang
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Abstract
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α-Glucosidase was immobilized onto an epoxyactivated resin (Eupergit C) to catalyze maltose into isomaltooligosaccharides (IMO), and then the effects of organic?aqueous media on the enzymatic properties of immobilized α-glucosidase were examined. An immobilization efficiency of 79.61% was obtained under the condition of pH 6.0, ionic strength of 2.0 M, and 30 mg of protein/g of resin. The butyl acetate-aqueous biphasic system was found to significantly improve the catalytic activity of the immobilized enzyme and the yield of IMO. The highest yield of IMO (50.83%, w/w) was obtained at pH 4.5 and 55 ℃ in a butyl acetate/buffer system (25:75, v/v). In addition, the immobilized enzyme particles were distributed into the organic phase after the completion of transglycosylation, which facilitates the separation and recycling use of the immobilized enzyme. Immobilized α-glucosidase retains a robust reusability in this continuous operation model. The present findings are of potential in improving the IMO manufacturing process.
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KEYWORD
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α-Glucosidase, Eupergit C, Immobilization, Isomaltooligosaccharides, Organic?aqueous media
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